Checking the calibration from zero is very important here. This is the most common mistake with calibration.
What happens is that TouchDRO has two offsets that it keeps internally: one from the scale zero to the absolute origin and another from absolute origin to incremental origin. Those are stored in microns. When you calibrate the scales, you change the ratio between scale encoder increments and microns, so the offsets change. This is why is VERY important to check the calibration from 0, as redvan22 did above.