Also got a couple of other projects done. One was adapting a flask holder to an orbital shaker for the lab, so we can use one of our incubators to grow bacterial cultures. Both parts were crazy cheap off Amazon, so it was worth the effort.
Second was spending 3 days at the beginning of the week with a very nice guy from Olympus to test drive a laser scanning confocal microscope that I'm rewriting a grant for. He brought an all singing all dancing version with him that is about $550,000, but we're asking for a simpler one for just $330,000
here's a mouse brain lesion. Blue = nuclei, green = actin (a cytoskeleton filament), not sure what red is labelling (colleagues work)
next up is an autofluorescence image of a fire worm (polychaete annelid) - the spikes are calciferous and break off in your skin causing either burning itching sensations or long term numbness
close up of the spikes (chitae?). My colleage was super excited by this image as some of the autofluorescence is seen only in the tips of the spikes, which might be the causative agent of the numbness. Long way down the road, but that might lead to new anaesthetics. Or not, given that it took over 18 months for the numbness in her finger tips to wear off when she was collecting them.
now my stuff:
a green fluorescent protein expressed in neurons (if you look closely you can see axons and dendrites)
a different construct expressed in the germ cell niches at the end of the worm gonads
and a final one expressed in the intestinal cells - the nuclei are the bright green circles
I love playing with microscopes, you can get some truly beautiful images