The software that came with the microscope has a calibration feature with additional features like drawing lines and shapes, annotating the image ,and the ability to measure angles and calculate areas of circles and rectangles. The calibration is in mm/pixel and seemed to be accurate. I haven't used it much, just a look-see. The software apparently only works with captured images, not the real time, and can be used with various image formats although the camera must be plugged in to open the software.
It works with a provided calibration grid with the crossed scales that I imaged, a series of different sized circles ranging from 100 microns to 1000 microns, a pattern of crossed lines on a 1 mm mesh size, line widths from .o3 to 1mm, inch and metric scales with .05 mm and .05in. divisions.
I am not really concerned about the calibration as both of my mills have a DRO and I intend to use the DRO to move the object under the crosshairs and obtain my coordinates from the DRO.
I have also used AutoCAD and SolidWorks for image analysis and I expect that other CAD packages offer similar capability.
Bob